Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue.
Identifieur interne : 001712 ( Main/Exploration ); précédent : 001711; suivant : 001713Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue.
Auteurs : Petra C. Boevink [Royaume-Uni] ; Paul R J. Birch ; Stephen C. WhissonSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Colorants fluorescents (métabolisme), Interactions hôte-pathogène (MeSH), Microscopie confocale (méthodes), Phytophthora (génétique), Phytophthora (métabolisme), Phytophthora (pathogénicité), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Tabac (microbiologie), Techniques de transfert de gènes (MeSH).
- MESH :
- génétique : Agrobacterium tumefaciens, Phytophthora, Protéines de fusion recombinantes.
- microbiologie : Tabac.
- métabolisme : Agrobacterium tumefaciens, Colorants fluorescents, Phytophthora, Protéines de fusion recombinantes.
- méthodes : Microscopie confocale.
- pathogénicité : Phytophthora.
- Interactions hôte-pathogène, Techniques de transfert de gènes.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Fluorescent Dyes (metabolism), Gene Transfer Techniques (MeSH), Host-Pathogen Interactions (MeSH), Microscopy, Confocal (methods), Phytophthora (genetics), Phytophthora (metabolism), Phytophthora (pathogenicity), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Tobacco (microbiology).
- MESH :
- chemical , genetics : Recombinant Fusion Proteins.
- chemical , metabolism : Fluorescent Dyes, Recombinant Fusion Proteins.
- genetics : Agrobacterium tumefaciens, Phytophthora.
- metabolism : Agrobacterium tumefaciens, Phytophthora.
- methods : Microscopy, Confocal.
- microbiology : Tobacco.
- pathogenicity : Phytophthora.
- Gene Transfer Techniques, Host-Pathogen Interactions.
Abstract
Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.
DOI: 10.1007/978-1-61737-998-7_16
PubMed: 21359810
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21359810</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>712</Volume><PubDate><Year>2011</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue.</ArticleTitle><Pagination><MedlinePgn>195-209</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-61737-998-7_16</ELocationID><Abstract><AbstractText>Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. 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Birch</name><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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